|The Microscope and how to use it
Dr. Georg Stehli
Publisher: Dover Pubns; ISBN: 0486225755; (June 1970)
|This book covers
apparatus care and use, simple (temporary) preparations of various kinds
of specimens, microphotography (though superficially), but the best part
is the appendix on microtome technique which presents a full procedure for
fixing, dehydration, and paraffin infiltration.
Here are the chapter titles:
1. The Microscope and Essential Tools
In its English translation, this book is a good introduction to microscopy and microtechnique but suffers from two problems:
1. The translation from the German is sometimes awkward and not always clear as to meaning (I have read the book in German as well);
2. The book makes reference to chemicals which cannot be obtained by private individuals in the US. Some chemical references are also no longer available: "wood vinegar", given by Dr. Stehli as a constituent of Pfeiffer's Fixative for algae, does not exist anywhere on this side of the Atlantic. Wood vinegar is actually a literal translation of the German "Holzessig" and is apparently an impure form of glatial acetic acid (which is available, but again not to individuals).
Students who have access to a college laboratory or who can order chemicals through a third party will be very happy with this book. The information on basic setup of a microscope, hand-cutting sections and preparation of fresh (as opposed to fixed) specimens is excellent.